| |
|
|
| |
|
|
Research  |
|
|
|
| |
| |
Genome-wide analysis of T-DNA integration into the chromosomes of Magnaporthe oryzae
|
|
|
|
|
|
| |
|
 Authours
|
Jaehyuk Choi, Jongsun Park, Junhyun Jeon, Myoung-Hwan Chi, Jaeduk Goh, Sung-Yong Yoo, Jaejin Park, Kyongyong Jung, Hyojeong Kim, Sook-Young Park, Hee-Sool Rho, Soonok Kim, Byeong Ryun Kim, Seong-Sook Han, Seogchan Kang, and Yong-Hwan Lee |
| Title |
Genome-wide analysis of T-DNA integration into the chromosomes of Magnaporthe oryzae |
| Journal |
Molecular Microbiology, 2007 (66) ~ |
Abstract
|
Agrobacterium tumefaciens-mediated transformation (ATMT) has become a prevalent tool for functional genomics of fungi, but our understanding of T-DNA integration into the fungal genome remains limited relative to that in plants. Using a model plant pathogenic fungus, Magnaporthe oryzae, here we report the most comprehensive analysis of T-DNA integration events in fungi and the development of an informatics infrastructure, termed a T-DNA analysis platform (TAP). We identified a total of 1,110 T-DNA-tagged locations (TTLs) and processed the resulting data via TAP. Analysis of the TTLs showed that T-DNA integration was biased among chromosomes and preferred the promoter region of genes. In addition, irregular patterns of T-DNA integration, such as chromosomal rearrangement and read-through of plasmid vectors were also observed, showing that T-DNA integration patterns into the fungal genome are as diverse as those of their plant counterparts. However, overall the observed junction structures between T-DNA borders and flanking genomic DNA sequences revealed that T-DNA integration into the fungal genome was more canonical than those observed in plants. Our results support the potential of ATMT as a tool for functional genomics of fungi and show that the TAP is an effective informatics platform for handling data from large-scale insertional mutagenesis. |
Link
|
 |
|
|
| |
|
|
|
|
| |
|
|
|
|
