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A putative MAP kinase kinase kinase, MCK1, is required for cell wall integrity and pathogenicity of the rice blast fungus, Magnaporthe oryzae
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 Authours
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Junhyun Jeon, Jaeduk Goh, Sungyong Yoo, Myoung-Hwan Chi, Jaehyuk Choi, Hee-Sool Rho,
Jongsun Park, Seong-Sook Han, Byeong Ryun Kim, Sook-Young Park, Soonok Kim, and
Yong-Hwan Lee |
| Title |
A putative MAP kinase kinase kinase, MCK1, is required for cell wall integrity and pathogenicity of the rice blast fungus, Magnaporthe oryzae |
| Journal |
Molecular Plant-Microbe Interactions, 2008 (21) ~ |
Abstract
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Insertional mutagenesis of Magnaporthe oryzae led to the
identification of MCK1, a pathogenicity gene predicted to
encode mitogen-activated protein kinase kinase kinase
(MAPKKK) homologous to BCK1 in Saccharomyces cerevisiae.
Targeted disruption of MCK1 resulted in the fungus
undergoing autolysis and showing hypersensitivity to cellwall-
degrading enzyme. The mck1 produced significantly
reduced numbers of conidia and developed appressoria in
a slightly retarded manner compared with the wild type.
Appressorium of the mck1 mutant was unable to penetrate
into plant tissues, thereby rendering the mutant nonpathogenic.
Cytorrhysis assay and monitoring of lipid mobilization
suggested that the appressorial wall was altered, presumably
affecting the level of turgor pressure within
appressorium. Furthermore, the mck1 mutant failed to
grow inside plant tissue. Complementation of the mutated
gene restored its ability to cause disease symptoms, demonstrating
that MCK1 is required for fungal pathogenicity.
Taken together, our results suggest that MCK1 is an
MAPKKK involved in maintaining cell wall integrity of M.
oryzae, and that remodeling of the cell wall in response to
host environments is essential for fungal pathogenesis. |
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