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Identification of Genes Encoding Heat Shock Protein 40 Family and the
Functional Characterization of Two Hsp40s, MHF16 and MHF21, in
Magnaporthe oryzae
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 Authours
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Mihwa Yi and Yong-Hwan Lee |
| Title |
Identification of Genes Encoding Heat Shock Protein 40 Family and the
Functional Characterization of Two Hsp40s, MHF16 and MHF21, in
Magnaporthe oryzae |
| Journal |
The Plant Pathology Journal, 2008 (24) ~ |
Abstract
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Magnaporthe oryzae, the causal agent of the rice blast
disease, poses a worldwide threat to stable rice
production. The large-scale functional characterization
of genes controlling the pathogenicity of M. oryzae is
currently under way, but little is known about heat
shock protein 40 (Hsp40) function in the rice blast
fungus or any other filamentous plant pathogen. We
identified 25 genes encoding putative Hsp40s in the
genome of M. oryzae using a bioinformatic approach,
which we designated M. oryzae heat shock protein forty
(MHF1-25). To elucidate the roles of these genes, we
characterized the functions of MHF16 and MHF21,
which encode type III and type II Hsp40 proteins,
respectively. MHF16 and MHF21 expression was not
significantly induced by heat shock, but it was downregulated
by cold shock. Knockout mutants of these
genes (¥Ämhf16 and ¥Ämhf21) were viable, but conidiation
was severely reduced. Moreover, sectoring was observed
in the ¥Ämhf16 mutant when it was grown on oatmeal
agar medium. Conidial germination, appressorium
formation, and pathogenicity in rice were not significantly
affected in the mutants. The defects in conidiation and
colony morphology were fully complemented by
reintroduction of wild type MHF16 and MHF21 alleles,
respectively. These data indicate that MHF16 and
MHF21 play important roles in conidiation in the rice
blast fungus. |
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