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MoSNF1 regulates sporulation and pathogenicity in the rice blast fungus
Magnaporthe oryzae
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 Authours
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Mihwa Yi, Jin-Hyoung Park, Joong-Hoon Ahn, Yong-Hwan Lee |
| Title |
MoSNF1 regulates sporulation and pathogenicity in the rice blast fungus
Magnaporthe oryzae |
| Journal |
FUNGAL GENET BIOL, 2008 (45) ~ |
Abstract
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The protein kinase Snf1 is a major component of the glucose derepression pathway in yeast and a regulator
of gene expression for the cell wall degrading enzyme (CWDE) in some plant pathogenic fungi. To
address the molecular function of Snf1 in Magnaporthe oryzae, which causes the rice blast disease, MoSNF1
was cloned and functionally characterized using gene knock-out strategies. MoSNF1 functionally
complemented the growth defect of the yeast snf1 mutant on a non-fermenting carbon source. However,
the growth rate of the Dmosnf1 mutant on various carbon sources was reduced independent of glucose,
and the expression of the CWDE genes in the mutant was induced during derepressing condition like the
wild type. The pre-penetration stage including conidial germination and appressorium formation of the
Dmosnf1 was largely impaired, and the pathogenicity of the Dmosnf1 was significantly reduced. Most
strikingly, the Dmosnf1 mutant produced only a few conidia and had a high frequency of abnormally
shaped conidia compared to the wild type. Our results suggest that MoSNF1 is a functional homolog of
yeast Snf1, but its contribution to sporulation, vegetative growth and pathogenicity is critical in M. oryzae. |
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