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Functional analysis of MCNA, a gene encoding a catalytic subunit of calcineurin,
in the rice blast fungus Magnaporthe oryzae
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 Authours
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Jinhee Choi, Yangseon Kim, and Yong-Hwan Lee |
| Title |
Functional analysis of MCNA, a gene encoding a catalytic subunit of calcineurin,
in the rice blast fungus Magnaporthe oryzae |
| Journal |
Journnal of Microbiology and Biotechnology, 2009 (19) ~ |
Abstract
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Magnaporthe oryzae, the causal agent of rice blast, forms a specialized infection structure, called an appressorium, which is crucial for penetration and infection of the host plant. Pharmacological data suggest that calcium/calmodulin-dependent signaling is involved in appressorium formation in this fungus. To understand the role of the calcium/calmodulin-activated protein phosphatase on appressorium formation at the molecular level, MCNA, a gene encoding the catalytic subunit of calcineurin, was functionally characterized in M. oryzae. Transformants expressing sense/antisense RNA of MCNA exhibited significant reductions in mycelial growth, conidiation, appressorium formation, and pathogenicity. cDNA of MCNA functionally complemented a calcineurin disruptant strain (cmp1::LEU2 cmp2::HIS3) of Saccharomyces cerevisiae. These data suggest that calcineurin A plays important roles in signal transduction pathways involved in infection-related morphogenesis and pathogenicity of M. oryzae. |
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