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A Quick and Safe Method for Fungal DNA Extraction
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 Authours
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Myoung-Hwan Chi, Sook-Young Park, Soonok Kim, and Yong-Hwan Lee |
| Title |
A Quick and Safe Method for Fungal DNA Extraction |
| Journal |
The Plant Pathology Journal, 2009 (25) ~ |
Abstract
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DNA-based studies, including cloning and genotyping, have become routine in fungal research laboratories. However, preparation of high-quality DNA from fungal tissue requires much time and labor and is often a limiting step for high-throughput experiments. We have developed a quick and safe (QS) DNA extraction method for fungi. Time efficiency and safety in the QS method were achieved by using plate-grown mycelia as the starting material, by eliminating organic extraction procedures, and by deploying a simple electric grinder. This QS method is applicable not only to a broad range of microbial eukaryotes, including true fungi and oomycetes, but also to lichens and plants. |
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