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The Global Response Regulator ExpA Controls Virulence Gene Expression through RsmA-Mediated and RsmA-Independent Pathways in Pectobacterium wasabiae SCC3193
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 Authours
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M. Broberg, G. W. Lee, J. Nykyri, Y. H. Lee, M. Pirhonen, E. T. Palva |
| Title |
The Global Response Regulator ExpA Controls Virulence Gene Expression through RsmA-Mediated and RsmA-Independent Pathways in Pectobacterium wasabiae SCC3193 |
| Journal |
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2014 (80) ~ |
Abstract
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ExpA (GacA) is a global response regulator that controls the expression of major virulence genes, such as those encoding plant
cell wall-degrading enzymes (PCWDEs) in the model soft rot phytopathogen Pectobacterium wasabiae SCC3193. Several studies
with pectobacteria as well as related phytopathogenic gammaproteobacteria, such as Dickeya and Pseudomonas, suggest that the
control of virulence by ExpA and its homologues is executed partly by modulating the activity of RsmA, an RNA-binding posttranscriptional
regulator. To elucidate the extent of the overlap between the ExpA and RsmA regulons in P. wasabiae, we characterized
both regulons by microarray analysis. To do this, we compared the transcriptomes of the wild-type strain, an expA mutant,
an rsmA mutant, and an expA rsmA double mutant. The microarray data for selected virulence-related genes were
confirmed through quantitative reverse transcription (qRT-PCR). Subsequently, assays were performed to link the observed
transcriptome differences to changes in bacterial phenotypes such as growth, motility, PCWDE production, and virulence in
planta. An extensive overlap between the ExpA and RsmA regulons was observed, suggesting that a substantial portion of ExpA
regulation appears to be mediated through RsmA. However, a number of genes involved in the electron transport chain and oligogalacturonide
metabolism, among other processes, were identified as being regulated by ExpA independently of RsmA. These
results suggest that ExpA may only partially impact fitness and virulence via RsmA. |
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