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Roles of Forkhead-box Transcription Factors in Controlling Development, Pathogenicity, and Stress Response in Magnaporthe oryzae
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 Authours
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Jaejin Park, Sunghyung Kong, Seryun Kim, Seogchan Kang and Yong-Hwan Lee |
| Title |
Roles of Forkhead-box Transcription Factors in Controlling Development, Pathogenicity, and Stress Response in Magnaporthe oryzae |
| Journal |
The Plant Pathology Journal, 2014 (30) ~ |
Abstract
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Although multiple transcription factors (TFs) have
been characterized via mutagenesis to understand their
roles in controlling pathogenicity and infection-related
development in Magnaporthe oryzae, the causal agent of
rice blast, if and how forkhead-box (FOX) TFs contribute
to these processes remain to be characterized. Four
putative FOX TF genes were identified in the genome of
M. oryzae, and phylogenetic analysis suggested that two
of them (MoFKH1 and MoHCM1) correspond to Ascomycota-
specific members of the FOX TF family while
the others (MoFOX1 and MoFOX2) are Pezizomycotina-
specific members. Deletion of MoFKH1 (ツMofkh1)
resulted in reduced mycelial growth and conidial
germination, abnormal septation and stress response,
and reduced virulence. Similarly, ツMohcm1 exhibited
reduced mycelial growth and conidial germination.
Conidia of ツMofkh1 and ツMohcm1 were more sensitive
to one or both of the cell cycle inhibitors hydroxyurea
and benomyl, suggesting their role in cell cycle control.
On the other hand, loss of MoFOX1 (ツMofox1) did not
show any noticeable changes in development, pathogenicity,
and stress response. Deletion of MoFOX2 was
not successful even after repeated attempts. Taken
together, these results suggested that MoFKH1 and Mo-
HCM1 are important in fungal development and that
MoFKH1 is further implicated in pathogenicity and
stress response in M. oryzae. |
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