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Role of MoAND1-mediated nuclear positioning in morphogenesis and pathogenicity in the rice blast fungus, Magnaporthe oryzae
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 Authours
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Junhyun Jeon˘Ó, Heekyoung Rho˘Ó, Seongbeom Kim, Kyoung Su Kim, Yong-Hwan Lee |
| Title |
Role of MoAND1-mediated nuclear positioning in morphogenesis and pathogenicity in the rice blast fungus, Magnaporthe oryzae |
| Journal |
FUNGAL GENETICS AND BIOLOGY, 2014 (69) ~ |
Abstract
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To cause disease on host plants, many phytopathogenic fungi undergo morphological transitions including
development of reproductive structures as well as specialized infection structures called appressoria.
Such morphological transitions display distinct nuclear dynamics. Here we report the developmental
requirement of MoAND1-mediated nuclear positioning for pathogenesis of the rice blast fungus, Magnaporthe
oryzae. The MoAND1 gene encodes a protein that shows high similarity to Num1 in Saccharomyces
cerevisiae and ApsA in Aspergillus nidulans, both of which are cell cortex proteins involved in nuclear
migration and positioning. Targeted deletion of MoAND1 did not affect radial growth of the fungus but
impaired nuclear distribution along the hyphae, which is reminiscent of ApsA mutant. In contrast to
the wild-type, which produces three to five spores in a sympodial manner on the conidiophore, only a
single spore was borne on the conidiophore of DMoand1, resulting in 65% decrease in conidia production,
compared to the wild-type. The mutant conidia displayed abnormalities in septation pattern and
nuclear distribution, which were correlated with their inability to germinate. Spores of the mutant that
did germinate were capable of differentiating appressoria but were defective in the execution of programmed
nuclear migration and positioning during development. Furthermore, mutant appressoria were
not fully functional, leading to delay in penetration of host plants. However, the ability of DMoand1 to
grow inside host tissues was comparable to that of the wild-type. All these defects greatly decreased
the virulence of the mutant. Taken together, our data suggest that there is a stringent but incomplete
developmental requirement for proper migration and positioning of fungal nuclei mediated by MoAND1
during asexual reproduction and pre-penetration phase of fungal pathogenesis. |
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