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A quick and accurate screening method for fungal gene-deletion mutants
by direct, priority-based, and inverse PCRs
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 Authours
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Jaejin Park, Seongbeom Kim, Seomun Kwon, Yong-Hwan Lee |
| Title |
A quick and accurate screening method for fungal gene-deletion mutants
by direct, priority-based, and inverse PCRs |
| Journal |
JOURNAL OF MICROBIOLOGICAL METHODS, 2014 (105) ~ |
Abstract
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Using two-step-PCR screening which consists of direct and priority-based PCR and inverse PCR, fungal genedeletion mutants were selected quickly and accurately. It omits genomic DNA extraction and Southern blotting steps and prevents misinterpretations caused by PCR failure. It is anticipated to facilitate large-scale reverse genetic studies in fungi. |
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