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Transcriptome Analysis of Early Responsive Genes in Rice during Magnaporthe oryzae Infection
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 Authours
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Yiming Wang† , Soon Jae Kwon†, Jingni Wu, Jaeyoung Choi, Yong-Hwan Lee, Ganesh Kumar Agrawal, Shigeru Tamogami, Randeep Rakwal, Sang-Ryeol Park, Beom-Gi Kim, Ki-Hong Jung, Kyu Young Kang, Sang Gon Kim* and Sun Tae Kim* |
| Title |
Transcriptome Analysis of Early Responsive Genes in Rice during Magnaporthe oryzae Infection |
| Journal |
PLANT PATHOLOGY JOURNAL, 2014 (30) ~ |
Abstract
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Rice blast disease caused by Magnaporthe oryzae is one
of the most serious diseases of cultivated rice (Oryza
sativa L.) in most rice-growing regions of the world.
In order to investigate early response genes in rice, we
utilized the transcriptome analysis approach using a
300 K tilling microarray to rice leaves infected with
compatible and incompatible M. oryzae strains. Prior
to the microarray experiment, total RNA was validated
by measuring the differential expression of rice defenserelated
marker genes (chitinase 2, barwin, PBZ1, and
PR-10) by RT-PCR, and phytoalexins (sakuranetin
and momilactone A) with HPLC. Microarray analysis
revealed that 231 genes were up-regulated (>2 fold
change, p < 0.05) in the incompatible interaction compared
to the compatible one. Highly expressed genes
were functionally characterized into metabolic processes
and oxidation-reduction categories. The oxidative
stress response was induced in both early and later
infection stages. Biotic stress overview from MapMan
analysis revealed that the phytohormone ethylene as
well as signaling molecules jasmonic acid and salicylic
acid is important for defense gene regulation. WRKY
and Myb transcription factors were also involved in signal
transduction processes. Additionally, receptor-like
kinases were more likely associated with the defense
response, and their expression patterns were validated
by RT-PCR. Our results suggest that candidate genes,
including receptor-like protein kinases, may play a key
role in disease resistance against M. oryzae attack. |
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