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Analysis of in planta Expressed Orphan Genes in the Rice Blast Fungus Magnaporthe oryzae
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 Authours
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Md. Abu Sadat˘Ó, Junhyun Jeon˘Ó, Albely Afifa Mir, Seongbeom Kim, Jaeyoung Choi and Yong-Hwan Lee |
| Title |
Analysis of in planta Expressed Orphan Genes in the Rice Blast Fungus Magnaporthe oryzae |
| Journal |
PLANT PATHOLOGY JOURNAL, 2014 (30) ~ |
Abstract
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Genomes contain a large number of unique genes which
have not been found in other species. Although the origin
of such ˇ°orphanˇ± genes remains unclear, they are
thought to be involved in species-specific adaptive processes.
Here, we analyzed seven orphan genes (MoSPC1
to MoSPC7) prioritized based on in planta expressed
sequence tag data in the rice blast fungus, Magnaporthe
oryzae. Expression analysis using qRT-PCR confirmed
the expression of four genes (MoSPC1, MoSPC2, Mo-
SPC3 and MoSPC7) during plant infection. However,
individual deletion mutants of these four genes did
not differ from the wild-type strain for all phenotypes
examined, including pathogenicity. The length, GC
contents, codon adaptation index and expression during
mycelial growth of the four genes suggest that these
genes formed during the evolutionary history of M. oryzae.
Synteny analyses using closely related fungal species
corroborated the notion that these genes evolved
de novo in the M. oryzae genome. In this report, we
discuss our inability to detect phenotypic changes in the
four deletion mutants. Based on these results, the four
orphan genes may be products of de novo gene birth
processes, and their adaptive potential is in the course
of being tested for retention or extinction through natural
selection. |
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