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Genome-wide profiling of DNA methylation provides insights into epigenetic regulation of fungal development in a plant pathogenic fungus, Magnaporthe oryzae
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Authours
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Junhyun Jeon, Jaeyoung Choi, Gir-Won Lee, Sook-Young Park, Aram Huh, Ralph A. Dean & Yong-Hwan Lee |
Title |
Genome-wide profiling of DNA methylation provides insights into epigenetic regulation of fungal development in a plant pathogenic fungus, Magnaporthe oryzae |
Journal |
SCI REP-UK, 2015 (5) ~ |
Abstract
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DNA methylation is an important epigenetic modification that regulates development of plants and
mammals. To investigate the roles of DNA methylation in fungal development, we profiled genome-wide
methylation patterns at single-nucleotide resolution during vegetative growth, asexual reproduction, and
infection-related morphogenesis in a model plant pathogenic fungus, Magnaporthe oryzae. We found that
DNA methylation occurs in and around genes as well as transposable elements and undergoes global
reprogramming during fungal development. Such reprogramming of DNA methylation suggests that it may
have acquired new roles other than controlling the proliferation of TEs. Genetic analysis of DNA
methyltransferase deletion mutants also indicated that proper reprogramming in methylomes is required
for asexual reproduction in the fungus. Furthermore, RNA-seq analysis showed that DNA methylation is
associated with transcriptional silencing of transposable elements and transcript abundance of genes in
context-dependent manner, reinforcing the role of DNA methylation as a genome defense mechanism. This
comprehensive approach suggests that DNA methylation in fungi can be a dynamic epigenetic entity
contributing to fungal development and genome defense. Furthermore, our DNA methylomes provide a
foundation for future studies exploring this key epigenetic modification in fungal development and
pathogenesis. |
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