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Potential roles of laccases on virulence of Heterobasidion annosum s.s.
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 Authours
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Hsiao-Che Kuo, Nicolas Detry, Jaeyoung Choi, Yong-Hwan Lee |
| Title |
Potential roles of laccases on virulence of Heterobasidion annosum s.s. |
| Journal |
MICROBIAL PATHOGENESIS, 2015 (81) ~ |
Abstract
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Laccases, multi-copper-containing proteins, can catalyze the oxidation of phenolic substrates and have
diverse functions such as a virulence factor in fungi. However, limited information can be found on the
role of laccases in the interaction of Heterobasidion annosum s.s. to its host plant. Due to genome
availability of the close-related species Heterobasidion irregulare, which contains 18 predicted laccaseencoding
genes, phylogenetic analysis and gene expression profiling were performed. Eighteen laccase
genes could be classified into 4 groups based on protein domains and phylogenetic analysis. However,
there is no clear indication between phylogeny and domain compositions in laccases, and lifestyles of
fungal species. The results of qRT-PCR showed that the expression of 8 laccase genes was highly upregulated
in Scots pine seedlings at 1 wpi. These data suggested that they might be involved in early
stage of host infection. In addition, up-regulation of gene expression under glucose condition as a sole
carbon source suggests that those laccases are not under carbon catabolite repression. Higher activities of
laccase were observed in culture media containing cellulose, sucrose, or glucose compared to that of
cellobiose as a sole carbon source. The highest mortality of Scots pine seedlings was observed when
infected by H. annosum s.s. on extra carbon source as glucose. This was supported by the facts that
glucose plays significant roles on up-regulation of laccase genes in planta and higher activity of laccase in
H. annosum s.s.. Taking all together, laccases in H. annosum s.s. have diverse functions and a group of
laccases may play a role during interactions with Scots pine seedlings. |
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