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Systematic characterization of the peroxidase gene family provides new insights into fungal pathogenicity in Magnaporthe oryzae
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 Authours
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Albely Afifa Mir, Sook-Young Park, Md. Abu Sadat, Seongbeom Kim, Jaeyoung Choi, Junhyun Jeon, and Yong-Hwan Lee |
| Title |
Systematic characterization of the peroxidase gene family provides new insights into fungal pathogenicity in Magnaporthe oryzae |
| Journal |
SCIENTIFIC REPORTS, 2015 (5) ~ |
Abstract
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Plants respond to microbial attack by rapid generation of reactive oxygen species (ROS) as part of their natural immunity. In response, fungal pathogens have evolved effective antioxidant defense systems. Recent studies proposed peroxidases as components of this defense system. The role of fungal peroxidases during interaction with host plants has not been explored at the genomic level. In this study, we systematically identified peroxidase genes and analyzed their impact on fungal pathogenesis in a model plant pathogenic fungus, Magnaporthe oryzae, which is responsible for the disease rice (Oryza sativa L.) blast. Phylogeny reconstruction placed 27 putative peroxidase genes into 15 clades. Expression profiles of peroxidase genes, using quantitative real-time PCR, showed that 23 genes were up regulated during plant infection and 15 genes were responsive, in vitro, to H2O2. Seven genes (MoAPX1, MoAPX2, MoCCP1, MoHPX1, MoLDS1, MoPRX2, and MoPRX1) were then selected from clades containing genes not characterized previously. Examination of individual deletion mutants of the seven genes revealed that these genes contribute to fungal development and/or pathogenesis. We identified significant and positive correlations among sensitivity to H2O2, peroxidase activity and fungal pathogenicity. In-depth analysis of MoPRX1 demonstrated that it is a functional ortholog of thioredoxin peroxidase in Saccharomyces cerevisiae and is required for detoxification of the oxidative burst within host cells. Transcriptional profiling of other peroxidases in ¥ÄMoprx1 supported this interwoven nature of the peroxidase-mediated, antioxidation defense system. The results from this study provide insight into the infection strategy built on evolutionarily conserved peroxidases in the rice blast fungus. |
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