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Genome-Wide Analysis of HypoxiaResponsive Genes in the Rice Blast Fungus, Magnaporthe oryzae
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 Authours
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Jaehyuk Choi☯, Hyunjung Chung☯, Gir-Won Lee, Sun-Ki Koh, Suhn-Kee Chae, Yong-Hwan Lee* |
| Title |
Genome-Wide Analysis of HypoxiaResponsive Genes in the Rice Blast Fungus, Magnaporthe oryzae |
| Journal |
PLOS ONE, 2015 (10) ~ |
Abstract
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Rice blast fungus, Magnaporthe oryzae, is the most destructive pathogen in the rice-growing
area. This fungus has a biotrophic phase early in infection and later switches to a necrotrophic
lifestyle. During the biotrophic phase, the fungus competes with its host for nutrients
and oxygen. Continuous uptake of oxygen is essential for successful establishment of blast
disease of this pathogen. Here, we report transcriptional responses of the fungus to oxygen
limitation. Transcriptome analysis using RNA-Seq identified that 1,047 genes were up-regulated
in response to hypoxia. Those genes are involved in mycelial development, sterol biosynthesis,
and metal ion transport based on hierarchical GO terms, and are well-conserved
among three fungal species. In addition, null mutants of two hypoxia-responsive genes
were generated and their roles in fungal development and pathogenicity tested. The mutant
for the sterol regulatory element-binding protein gene, MoSRE1, exhibited increased sensitivity
to a hypoxia-mimicking agent, increased conidiation, and delayed invasive growth
within host cells, which is suggestive of important roles in fungal development. However,
such defects did not cause any significant decrease in disease severity. The other null
mutant, for the alcohol dehydrogenase gene MoADH1, showed no defect in the hypoxiamimicking
condition (using cobalt chloride) and fungal development. Taken together, this
comprehensive transcriptional profiling in response to a hypoxic condition with experimental
validations would provide new insights into fungal development and pathogenicity in plant
pathogenic fungi. |
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