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FUSARIUM-ID v.3.0: An Updated, Downloadable Resource for Fusarium Species Identification
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 Authours
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Terry J. Torres-Cruz1
Briana K. Whitaker2
Robert H. Proctor2
Kirk Broders2
Imane Laraba2
Hye-Seon Kim2
Daren W. Brown2
Kerry O¡¯Donnell2
Tania L. Estrada-Rodríguez3
Yong-Hwan Lee4 5
Kyeongchae Cheong5
Emma C. Wallace1
Chyanna T. McGee1
Seogchan Kang1
David M. Geiser1 ¢Ó |
| Title |
FUSARIUM-ID v.3.0: An Updated, Downloadable Resource for Fusarium Species Identification |
| Journal |
PLANT DISEASE, 2022 (106) ~ |
Abstract
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Species within Fusarium are of global agricultural, medical, and food/feed safety concern and have been extensively characterized. However, accurate identification of species is challenging and usually requires DNA sequence data. FUSARIUM-ID (http://isolate.fusariumdb.org/blast.php) is a publicly available database designed to support the identification of Fusarium species using sequences of multiple phylogenetically informative loci, especially the highly informative ¡680-bp 5¡Ç portion of the translation elongation factor 1-alpha (TEF1) gene that has been adopted as the primary barcoding locus in the genus. However, FUSARIUM-ID v.1.0 and 2.0 had several limitations, including inconsistent metadata annotation for the archived sequences and poor representation of some species complexes and marker loci. Here, we present FUSARIUM-ID v.3.0, which provides the following improvements: (i) additional and updated annotation of metadata for isolates associated with each sequence, (ii) expanded taxon representation in the TEF1 sequence database, (iii) availability of the sequence database as a downloadable file to enable local BLAST queries, and (iv) a tutorial file for users to perform local BLAST searches using either freely available software, such as SequenceServer, BLAST+ executable in the command line, and Galaxy, or the proprietary Geneious software. FUSARIUM-ID will be updated on a regular basis by archiving sequences of TEF1 and other loci from newly identified species and greater in-depth sampling of currently recognized species. |
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