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Nuclear localization sequence of MoHTR1, a
Magnaporthe oryzae effector, for
transcriptional reprogramming of immunity
genes in rice
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 Authours
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You-Jin Lim 1,2, Yoon-Ju Yoon2, Hyunjun Lee2, Gobong Choi3, Seongbeom Kim2,
Jaeho Ko2, Jea Hyeoung Kim4, Ki-Tae Kim 4,5 & Yong-Hwan Lee 1,2,3,6,7,8 |
| Title |
Nuclear localization sequence of MoHTR1, a
Magnaporthe oryzae effector, for
transcriptional reprogramming of immunity
genes in rice |
| Journal |
NAT COMMUN, 2024 (15) ~ |
Abstract
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Plant pathogens secrete nuclear effectors into the host nuclei to modulate
the host immune system. Although several nuclear effectors of fungal
pathogens have been recently reported, the molecular mechanism of NLSassociated
transport vehicles of nuclear effectors and the roles of NLS in
transcriptional reprogramming of host immunity genes remain enigmatic.
We previously reported the MoHTR1, a nuclear effector of the rice blast
fungus, Magnaporthe oryzae. MoHTR1 is translocated to rice nuclei but not
in fungal nuclei. Here, we identify the core NLS (RxKK) responsible for
MoHTR1¡¯s nuclear localization. MoHTR1 is translocated in the host nucleus
through interaction with rice importin ¥á. MoHTR1 NLS empowers it to
translocate the cytoplasmic effectors of M. oryzae into rice nuclei. Furthermore,
other nuclear effector candidates of the blast pathogen and rice
proteins which have RxKK also exhibit nuclear localization, highlighting the
crucial role of RxKK in this process. We also unveil the importance of
SUMOylation in the stability of MoHTR1 and translocation of MoHTR1 to
host nuclei. Moreover, MoHTR1 NLS is essential for the pathogenicity of M.
oryzae by reprogramming immunity-associated genes in the host. Our
findings provide insights into the significance of plant-specific NLS on fungal
nuclear effectors and its role in plant-pathogen interactions. |
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