|
|
|
|
|
Research |
|
|
|
|
|
Identification and characterization of new Copia-like retrotransposon Ors1 in rice.
|
|
|
|
|
|
|
|
Authours
|
Nam-Soo Jwa[0], Sook-Young Park[1], Chan-Ho Park[1], Seong-Sook Han[2] and Yong-Hwan Lee[1][*] |
Title |
Identification and characterization of new Copia-like retrotransposon Ors1 in rice. |
Journal |
The Plant Pathology Journal, 2003 (19) ~ |
Abstract
|
An insertion sequence identified as a solo long terminal
repeat (LTR) of a new rice copia-like retrotransposon
was detected in the ORF of the Pi-b gene from the rice
cv. Nipponbare, and was designated as Osr1. Osr1
consists of a 6386 bp nucleotide sequence including 965
bp LTRs on both ends with an 82% nucleotide sequence
identity to the wheat Tar1 retrotransposon on reverse
transcriptase. Nucleotide divergence was noted among
the individual LTRs, as well as the coding region of
Osr1. Various restriction fragment length polymorphism
(RFLP) of LTR were detected in indica cultivars,
whereas, only a few could be detected in the japonica
cultivars. The population of Osr1 is lower in the wildtype
rice compared with that in the domesticated
cultivars. The insertion of LTR sequence in the Pi-b
gene in the susceptible cultivar suggested that retrotransposon-
mediated insertional mutation might play
an important role in the resistance breakdown, as well
as in the evolution of resistance genes in rice. |
Link
|
|
|
|
|
|
|
|
|
|
|
|
|
|